Tip Sealing (TSipTM) for UHPLC

Some of the features of a fitting which implement the  TSiptm method are:

1. The fitting is configured to fit into a standard 10-32 HPLC port, also knows as a 'CPI port', or commonly, a 'column port'.  TSipTM  fits ALL of these ports regardless of manufacturer. 

2. There is no traditional cone shaped ferrule, or if a cone shaped ferrule is present, it is not the primary sealing mechanism.  So, the sample carry-over and dispersion associated with these volumes below the ferrule is bypassed.

3. There is a malleable seal located in such a position as to be manipulated by the fitting body to seal at the extreme bollom of the port, beyond the traditional tapered ferrule sealing area.  Again, the sample carry-over and dispersion associated with the volumes below the ferrule is bypassed.

4. There is a secondary tube which adapts the diamater of the smaller instrument-to-instrument tube to the larger diameter of the bottom of the port.  This tube does a lot for the design.  It provides two separate mechanisms to grasp the internal tubing such that it does not slip, even at UHPLC pressures.

5. This secondary tube, in conjunction with the body, forces pressure to be applied to the malleable seal, which creates a seal between the inner tubing and the bottom of the port.  This is an essential  secondary means of holding the tubing securely. 

These are just some of the advantages of this method.  But if one more might be mentioned, TSipTM fittings are easy to install and require no real effort or diligence on the part of the user to acheive lower variance in resolution.  Dispersion variance can arise from a number of factors.   Some of these factors are: port machining tolerance variation, user installation variation (seating depth and tightness) , ferrule and tubing wear and damage variation, and others.    Again, these variances are easily minimized using the TSipTM method.


With patents approved, now is the time for widespread replacement of existing fitting technologies with Tip Sealing type fittings.    The Tip Sealing method was designed to be used directly with existing instrumentation.  It is a technique, a method in USPTO parlance, rather than a specific apparatus.  This means the particular apparatus can come from a wide variety of licensed manufacturers, and in a wide variety of forms.   These variations of apparatus merely allow the user to implement the Tip Sealing method described in US patents 9217522, and as disclosed in earlier filings 8006367 and 8887371.

Typical apparatus used to acheive Tip Seal

A variety of physical implementations are possible.

A patented method of sealing a 10-32 HPLC port.

US patent 9,217,522 and others describe a method (not specific apparatus) for sealing a 10-32 HPLC port, also called a 'CPI port' in some circles.   The term "Tip Sealing" was coined to describe the method in US patents in 2007.  For several decades, fittings for HPLC ports consisted of better and better ways to seal with the traditional cone shaped ferrule.  Better cone shaped ferrules evolved as well.   The "Tip Sealing" (or TSipTM) method brings new life to the traditional HPLC port making it suitable for UHPLC use.   It does this by eliminating the cone shaped ferrule and the associated extra-column dispersion.  The TSipTM method can be implemented in a variety of physical forms, some allowing rather high pressure operation (15-25,000 PSI, perhaps greater).


Since the method is patented, users who wish to acheive the advantages of the TSipTM method should check that the apparatus they use is licensed by the inventor.