Tip Sealing (TSipTM) for UHPLC

Some of the features of a fitting which implement the  TSiptm method are:

1. The fitting is configured to fit into a standard 10-32 HPLC port, also knows as a 'CPI port', or commonly, a 'column port'.  TSipTM fits ALL of these ports regardless of manufacturer. 

2. There is no traditional cone shaped ferrule, or if a cone shaped ferrule is present, it is not the primary sealing mechanism.  So, the sample carry-over and dispersion associated with these volumes below the ferrule is bypassed.

3. There is a malleable seal located in such a position as to be manipulated by the fitting body to seal at the extreme bollom of the port, beyond the traditional tapered ferrule sealing area.  Again, the sample carry-over and dispersion associated with the volumes below the ferrule is bypassed.

4. There is a secondary tube which adapts the diamater of the smaller instrument-to-instrument tube to the larger diameter of the bottom of the port.  This tube does a lot for the design.  It provides two separate mechanisms to grasp the internal tubing such that it does not slip, even at UHPLC pressures.

5. This secondary tube, in conjunction with the body, forces pressure to be applied to the malleable seal, which creates a seal between the inner tubing and the bottom of the port.  This is an essential  secondary means of holding the tubing securely. 




These are just some of the advantages of this method.  But if one more might be mentioned, TSipTM fittings are easy to install and require no real effort or diligence on the part of the user to acheive low variance in resolution.


With patents approved, now is the time for widespread replacement of existing fitting technologies with Tip Sealing type fittings.    The Tip Sealing method was designed to be used directly with existing instrumentation.  It is a technique, a method in USPTO parlance, rather than a specific apparatus.  This means the particular apparatus can come from a wide variety of licensed manufacturers, and in a wide variety of forms.   These variations of apparatus merely allow the user to implement the Tip Sealing method described in US patent 9217522, and to a lesser extent, 8006367 and 8887371.


So, we say with as much confidence as possible at this stage:  The Tip Sealing method patents (TSipTM) will be the predominant means of making fluidic connections in UHPLC systems, and very likely HPLC systems in the near future.   This technology will ramp up to an appropriate volume for commodity level manufacturing. 


So how big will the market be?  Presently, we are gathering data to justify a ramp up in production.  Though a 'gut feel' for the size and impact of this fitting technology is positive, we seek input from manufacturers who are in the HPLC/UHPLC business and who may wish to participate through licensing, or OEM relationships.   Accuracy in sizing the market will ultimately allow manufaturers with an interest in this technology to obtain product at the lowest cost of manufacture.  Feel free to reach out and make contact so relationship building can begin early in the process.    



The Tip Sealing method for UHPLC.

The impact of many new technologies are often difficult to predict.   The Tip Sealing method for UHPLC appears to be a technology which will have a very positive impact on attaining the goals of UHPLC. 



  • Speed increase by a factor of 10
  • Solvent consumption is drastically reduced by up to 90%
  • Excellent peak shapes.
  • Enhanced sensitivity.


A typical embodiment of a TSipTM fitting........